Effect of Drought Stress on Some Growth Parameters and Several Biochemical Aspects in Two Pumpkin Species

Document Type : Original Article

Authors

1 Department of biology, Faculty of Agriculture, Islamic Azad University, Saveh Branch, Saveh, Iran

2 Department of Biology, Islamic Azad University, Gorgan Branch, Gorgan, Iran

3 M.Sc. in plant physiology, Payame Noor University, Tehran, Iran

10.22034/ijpp.2021.683057

Abstract

For considering the effect of drought stress on some morphological and biochemical changes in two pumpkin species, Cucurbita maxima L. and Cucurbita pepo L. a kind of experiment was done by field culture in three water dispersal levels with field capacity, 2/3 field capacity, and 1/3 field capacity, based on the factorial design in random block form with four replications. The results indicated that increasing the stress level, leaves water potential under drought stress decreased in comparison to the control sample in both species. But with increasing drought stress, root length increased too. Also during drought stress, root soluble carbohydrates content, ascorbic acid content, dehydroascorbic acid, catalase, polyphenol oxidase, and peroxidase enzymes activity increased significantly in 5% level, according to the results with increasing the stress, soluble carbohydrates content decreased in leaf.

Keywords


Alvarez-Robles M. J., M.P. Bernal, A.S anchez-Guerrero, F. Sevilla and R. Clemente.2020. 'Major As species, lipid peroxidation and protein carbonylation in rice plants exposed to increasing As(V) concentrations'. Heliyon 6: e04703.
Anjum, S.A., Xie, X.Y., Wang, L.C., Saleem, M.F., Man, C. and Lei, W. 2011.Morphological, phys-iological and biochemical responses of plants to drought stress. Afr. J. Agric. Res. 6,2026–2032.
Babazadeh Darjazi, B., M. Farzami Sepehr and B. Golein. 2019. 'The effect of rootstocks on sugars, acids, carotenoids, chlorophylls, and ethylene of Satsuma mandarin (Citrus unshiu)'. Iranian Journal of Plant Physiology ,10(1), 2999- 3008
Balakrishman,V., K. Venkatesan, C.Ravindran and G. Kulandaivelu. 2005.' Protective mechanism in UV-B treated Crotalaria juncea L. seedlings'. Plant Protect. Sci., 41, 3: 115–120.
 Biswas, D. A. Mukherjee, D. Kumar Pandey and A. Dey. 2021. 'Aquaporins and their regulation for osmotic adjustment of plants', Editor(s): Aryadeep Roychoudhury, Durgesh Kumar Tripathi, Rupesh Deshmukh. Transporters and Plant Osmotic Stress. Academic Press, 365-381,
Bohnert, H. J. and R. G. Jensen. 1996. 'Strategies for engineering water- stress tolerance in plants'.  Tibtech,  14: 89-97.
Boyer J. S. 1967. 'Leaf Water Potentials Measured with a Pressure Chamber'. Plant Physiology, 42( 1): 133–137.
Cassia, R., M. Nocioni, N. Correa-Aragunde and Lamattina.2018.' Climate change and the impact of greenhouse gasses: CO2 and NO, friends and foes of plant oxidative stress'. Front. Plant Sci. 9 : 273.
 Chang, L., L. Wang, C. Peng, Z. Tong, D. Wang, G. Ding, J. Xiao, A. Guo and X. Wang. 2019. 'The chloroplast proteome response to drought stress in cassava leaves'. Plant Physiology and Biochemistry, 142:351-362.
Chaturvedi, A. K., U. Surendran, G. Gopinath, K. M. Chandran,  NK. Anjali and CT. Mohamed Fasil .2019. 'Elucidation of stage specific physiological sensitivity of okra to drought stress through leaf gas exchange, spectral indices, growth and yield parameters'. Agricultural Water Management, 222: 92-104.
Chassemi, F., A. J. Jakeman and M. A. Nix. 1995. 'Stalinization of land and water resources'. University of  New South Wales Press LTD.
Choudhury, S. and S. K. Panada. 2004. 'Role of salicylic acid regulating cadmium induced oxidative stress in (Oryza sativa L. ) roots'. Plant Physiology, 30:95-110.
Dawood, M.F.A.; M. Tahjib-Ul-Arif, A. A. M. Sohag, A. A. H. Abdel Latef and M. M.  Ragaey. 2021. 'Mechanistic Insight of Allantoin in Protecting Tomato Plants Against Ultraviolet C Stress'. Plants, 10, 11.
Daryanto, S.  L. Wang and P.A. Jacinthe. 2017.'Global synthesis of drought effects on cereal, legume, tuber and root crops production: a review'. Agric. Water Manag., 179 : 18-33.
Debolt, S., V. Melino and C.M. Ford. 2007. 'Ascorbate as a Biosynthetic Precursor in Plants'. Annals of  Botany , 99: 3–8.
El -Tayeb , M .A. 2005.' Response of barley grains to the interactive effect of Salinity and salicylic  acid'. Plant Growth Regulation, 45: 215-225.
Farzamisepehr, M. and F. Norouzi. 2018. 'Physiological responses of Polypogon monspeliensis L. in petroleum-contaminated soils'. Iranian Journal of Plant Physiology 8 (2), 2391-2401.
Fekia , K. ,Y. Kamoun, R. Ben Mahmoud, A. Farhat-Khemakhem, A. Gargouri and F. Brinia. 2015. 'Multiple abiotic stress tolerance of the transformants yeast cells and the transgenic Arabidopsis plants expressing a novel durum wheat catalase'. Plant Physiology and Biochemistry,97: 420-431.
Jaskulak,M.  A. Grobelak, A. Grosser and  F. Vandenbulcke. 2019. Gene expression, DNA damage and other stress markers in Sinapis alba L. exposed to heavy metals with special reference to sewage sludge application on contaminated sites, Ecotoxicology and Environmental Safety,181,:508-517
Jiang, Y.  and  B. Huang.2001. 'Drought and Heat stress injure to two cool-season turf grass in relation to Antioxidant Metabolism and Lipid peroxitation'. Crop Sci, 41: 436-442.
Kar, M.  and D. Mishra. 1976. 'Catalase, Peroxidase, and Polyphenoloxidase Activities during Rice Leaf Senescence'. Plant Physiology, 57(2): 315–319.
Kobayashi, Y., Y. Nojima, T. Sakamoto, K. Iwabuchi, T. Nakazato, H. Bono, A. Toyoda, A. Fujiyama, M. R. Kanost and H. Tabunoki. 2019. 'Comparative analysis of seven types of superoxide dismutases for their ability to respond to oxidative stress in Bombyx mori.' Sci. Rep. 9, 1–12.
Kochert, G. 1978. 'Carbohydrate determination by the phenol sulfuric acid method'. In: Helebust. J. A. Craig, J. S.(ed): Hand book of Phycological Method, pp. 56-97. Cambridge Univ. Press. Cambridge.
Kowitcharoen L. , C. Wongs-Aree, S. Setha, R. Komkhuntod, V. Srilaong and S. Kondo .2015. 'Changes in abscisic acid and antioxidant activity in sugar apples under drought conditions'. Sci. Hortic. 193: 1-6.
Kowitcharoen L. , C. Wongs-Aree, S. Setha, R. Komkhuntod, V. Srilaong and S. Kondo .2017. 'Physiological changes of fruit and C/N ratio in sugar apples (Annona squamosa L.) under drought conditions. Acta Hortic. 1166, 195-202.
Kowitcharoen L. , C. Wongs-Aree, S. Setha, R. Komkhuntod, S. Kondo and  V. Srilaong.'2018. Pre-harvest drought stress treatment improves antioxidant activity and sugar accumulation of sugar apple at harvest and during storage'. Agriculture and Natural Resources,52 :146-154.
Kumar,U., M. Kaviraj, P. Panneerselvam, H. Priya, K. Chakraborty, P. Swain, S.N. Chatterjee, S.G. Sharma, P.K. Nayak and  A.K. Nayak. 2019. 'Ascorbic acid formulation for survivability and diazotrophic efficacy of Azotobacter chroococcum Avi2 (MCC 3432) under hydrogen peroxide stress and its role in plant-growth promotion in rice (Oryza sativa L.)'. Plant Physiology and Biochemistry, 139: 419-427.
Kusaka, M., A. G. Lalusin and T. Fujimura. 2005. 'The maintenance of growth and turgor in pearl millet (Pennisetum glaucum L.) cultivars with different root structures and osmo-regulation under drought stress'. Plant Science,  168: 1-14.
Laxa, M., M. Liebthal, W. Telman, K. Chibani and  K. J. Dietz. 2019. 'The role of the plant anti-oxidant system in drought tolerance'. Antioxidants. 8, 1–14.
Lee, M. T. and B. H. Shiu. 2002. 'Stability of lycopene during heating and illumination in a model system'. Food Chem, 78: 425-432.
Lindsay, C.A., 1988. 'Irrigation Scheduling of Subsurface Drip Irrigated Salad Tomatoes'. M.Sc. Agr Thesis, University of Sydney, N.S.W., 150 pp.
Liptay, A., P. Sikkema and W. Fonteno. 1998. 'Transplant growth control through water deficit stress-a, Review'. Hort. Technol, 8:  1-4.
Liu H.,W.  Jiang ,Y. Bi  and Y. Luo. 2005. 'Postharvest BTH treatment induces resistance of peach (Prunus persica L. cv. Jiubao) fruit to infection by Penicillium expansumand enhances activity of fruit defense mechanisms'. Postharvest Biology and Technology, 35: 263–269.
Maleki , M., A. Shojaeiyana and A. Mokhtassi-Bidgoli .2021. 'Genotypic variation in biochemical and physiological responses of fenugreek (Trigonella foenum-graecum L.) landraces to prolonged drought stress and subsequent rewatering'.Scientia Horticulturae, 287.
Niinemets, Ü. 2010. 'A review of light interception in plant stands from leaf to canopy in different plant functional types and in species with varying shade tolerance'. Ecological Research 25(4), 693-714.
Orsavová J, I. Hlaváčová, J. Mlček, L. Snopek and L. Mišurcová . 2019. 'Contribution of phenolic compounds, ascorbic acid and vitamin E to antioxidant activity of currant (Ribes L.) and gooseberry (Ribes uva-crispa L.) fruits'. Food Chemistry. 284:323-333.
Ouledali , Z., M. Ennajeha,A. Ferrandinob, H. Khemira, A. Schubertb and F. Secchi. 2019. 'Influence of arbuscular mycorrhizal fungi inoculation on the control of stomata functioning by abscisic acid (ABA) in drought-stressed olive plants'. South African Journal of Botany ,121 :152–158.
Osama, S., M. El Sherei, D. A. Al-Mahdy, M. Bishr and O. Salama.2019. 'Effect of Salicylic acid foliar spraying on growth parameters, γ-pyrones, phenolic content and radical scavenging activity of drought stressed Ammi visnaga L. plant'. Industrial Crops and Products,134: 1-10.
Özenç, D.B. 2008. 'Growth and transpiration of tomato seedlings grown in Hazelnut Husk compost under water-deficit stress'. Compost Science & Utilization 16(2), 125-131.
Pastori G. M. and V. S.  Trippi. 1993. 'Cross resistance between water and oxidative stress in wheat leaves'. J. agri. Sci, 120: 289-294.
Pinto, M.C., D. Francis and L. De Gara, L.1999. 'The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells'. Protoplasma 209, 90–97 .
Peleg, Z.  and E. Blumwald. 2011. 'Hormone balance and abiotic stress tolerance in crop plants'. Current Opinion in Plant Biology ,14(3), 290-295.
Ripoll, J., L. Urban, M. Staudt, F. Lopez-Lauri, L.P.R. Bidel and N.  Bertin. 2014. 'Water shortage and quality of fleshy fruits - making the most of the unavoidable'. J. Exp. Bot. 65, 4097-4117.
Rosales, M. A., J. M. Ruiz, J.  Hernandez, T.  Soriano, N. Castilla and L.  Romero. 2006. 'Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation'. J. Sci Food Agric, 86: 1545-1551.     
Shanker, R. and P. Sharma. 2005. 'Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedling'. Journal of Plant and Growth Regulation, 46: 209–221.
Sunkar, R, D. Bartels and H. H. Kirch.2003. 'Over expression of a stress inducible dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance'. Plant Journal, 35(4):452-64.
Synerri, C. L. M., C. Pizino and  F. N. Avari –Izzo.1993. 'Chemical change and O2 production in thylakoid memberanes under water stress'. Plant Physiol,  87: 211-216.
Thipyapong P, MJ. Stout  and J.  Attajarusit. 2007. 'Functional analysis of polyphenol oxidases by antisense/sense technology'. Molecules (Basel, Switzerland), 12(8):1569-1595.
Tuladhar P. S. Sasidharan and P. Saudagar.2021. 'Role of phenols and polyphenols in plant defense response to biotic and abiotic stresses'. Biocontrol Agents and Secondary Metabolites , Applications and Immunization for Plant Growth and Protection. Woodhead Publishing, Elsevier Inc.  Pages 419-441.
Waśkiewicz A, M. Beszterda and P. Goliński.2014. 'Nonenzymatic antioxidants in plants'. In oxidative damage to plants. Academic Press, pp. 201-234.
Watkinson, J. I., L. Hendricks, A. A. Sioson, C. Vasquez-Robinet, S. Verlyn, L. S. Heath, M. Schuler, H. J. Bohnert, M. Bonierbale and R. Grene. 2006. 'Accessions of Solanum tuberosum ssp. indigena show differences in photosynthetic recovery after drought stress as reflected in gene expression profiles'. Plant Science,  19: 1-14.
Zhang, J. and  X.  Sun. 2021. 'Recent advances in polyphenol oxidase-mediated plant stress responses'.  Phytochemistry, 181:112588.