Essential oil Storage conditions affect the chemical composition in cultivated Mentha spicata

Document Type : Original Article


1 1Department of Horticultural Science, Yasooj Branch, Islamic Azad University, Yasooj, Iran

2 DepartmentofAgriculture,PayameNoor University,Tehran,Iran

3 Department of Chemistry, Yasooj Branch, Islamic Azad University, Yasooj, Iran.



Spearmint (Mentha spicata) is widely utilized in traditional medicine and folk remedies. The fresh and dried plants and their essential oils are widely used in food, cosmetics, confectionery, chewing gum, toothpaste, and pharmaceutical industries. In this research, the essential oil of air-dried samples was obtained by hydro-distillation and was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Changes in essential oil compositions were detected during storage for 3 months in refrigerator (4 ◦C), freezer (−20 ◦C), and at room temperature (25 ◦C). The results indicated that at room temperature, the proportions of the compounds with lower boiling temperatures such as 1,8-Cineole (3.78-3.34%), Limonene (7.33-6.61%), and Germacrene D (6.26-1.90%) were decreased significantly. Furthermore, the essential oil composition showed the least alterations and Mentha spicata L. kept its primary quality when stored at low temperatures, particularly in the freezer, and carvone had an increased significantly in all of the treatments


Bimakr, M., R. A. Rahman, F. S.Taip, A. Ganjloo  and L. M. Salleh, 2011. 'Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves'. Food and Bioproducts Processing, 89: 67-72.
 Burt, S,  2004. 'Essential oils: their antibacterial properties and potential applications in foods- a review'. International Journal of Food Microbiology, 94(3): 223-253.
Demuner, A.J.,  L. C. Almeida Barbosa,  C. Gonçalves Magalhaes,  C. J. Da Silva,  C. R. Ghelardini, C.,  N. Galeotti  and  G. Mazzanti, 2001. ‘Local anaesthetic activity of monoterpenes and phenylpropanes of essential oils'. Planta Medica, 67(06): 564-566.
Gulluce, M.,  F. Sahin,  M. Sokmen,  H. Ozer and D. Daferera, 2007.'Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. Longifolia'. Food Chemistry, 103: 1449-1456.
Leporatti,  M. L and  K. Ghedira. 2009. ' Comparative analysis of medicinal plants used in traditional medicine in Italy and Tunisia'. Journal of Ethnobiology and Ethnomedicine, 5: 31–39.
Miguel, G.,  M. Simoes, A. C. Figueiredo, J. G. Barroso,  L. G. Pedro, L.G and L. Carvalho. 2004. 'Composition and antioxidant activitiesof the essential oils of Thymus caespititius, Thymus camphoratus and Thymus mastichina'. Food Chemistry, 86(2): 183-188.
Najafian, SH. 2014. 'Storage conditions affect the essential oil composition of cultivated Balm Mint Herb (Lamiaceae) in Iran'. Industrial Crops and Products, 52: 575-581.
Najafian, SH. 2016. 'The effect of time and temperature on the shelf life of essential oils of Lavandula officinalis'. Journal of Essential oil Research, 28(5): 413-420.
Rowshan, V.,  A. Bahmanzadegan and  M. J. Saharkhiz. 2013. ' Influence of storage conditions on the essential oil composition of Thymus daenensis Celak'. Industrial Crops and Products, 49: 97-101.
Sanchez, E.,  S.García and  N. Heredia.  2010. ' Extracts of edible and medicinal plants damage membranes of Vibrio cholerae'. Applied and Environmental Microbiology, 76(20): 6888-6894.
Sawamura, M.,  U. S. Son, H. S. Choi,  M. S. Kim, N. T. L, Phi, M. Fears  and  C. Kumagai. 2004. 'Compositional changes in commercial lemon essential oil for aromatherapy'. International Journal of Aromatherapy, 14(1): 27-36.
Tajkarimi, M.M.,  S. A. Ibrahim  and D. O. Cliver. 2010. ' Antimicrobial herb and spice compounds in food'. Food Control, 21(9): 1199-1218.
Tawaha, K., F.Q. Alali,  M. Gharaibeh,  M. Mohamed and T. El Elimat. 2007. 'Antioxidant activity and total phenolic content of selected Jordanian plant species'. Food Chemistry, 104: 1372–1378.
Tetika, F., S. Civelek and U. Cakilcioglu. 2013. ' Traditional uses of some medicinal plants in Malatya (Turkey) '. Journal of Ethnopharmacology, 146: 331- 346.
Usai, M.,  M. Marchetti, M. Foddai,  A. Del Caro, R. Desogus,  I. Sanna  and A. Piga. 2011. ' Influence of different stabilizing operations and storage time on the composition of essential oil of thyme (Thymus officinalis L.) and rosemary (Rosmarinus officinalis L.) '. LWT-Food Science and Technology, 44(1): 244-249.