The Role of Potassium and Ascorbic acid on Some Growth and Physiological Responses in Catharanthus roseus

Document Type : Original Article

Authors

1 Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.

2 Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

10.22034/ijpp.2021.681098

Abstract

Different concentrations (1.5, 3.16, 15, and 30 mM) and forms (K2SO4 and KNO3) of potassium applied through Hoagland’s nutrient solution. Ascorbic acid in concentrations of 750 and 1500 mg L-1 sprayed twice on seedlings (age 68 and 78 days). Wet and dry weight, plant height, photosynthetic pigments, sodium, and potassium contents measured at the end of the growing season. Also, total free amino acids measured by HPLC. The excess of potassium increased the total wet (62%) and dry weight (54%), leaf area (31%), shoot and root height (49% and 15%, respectively), total chlorophyll (44%), and K+/Na+ ratio (100%), while the total free amino acids (2 times) and sodium content (28%) decreased. Ascorbic acid showed an almost similar trend to potassium in the mentioned factors, but it did not affect the root height and total sodium and potassium contents. There was a positive interaction between the potassium and ascorbic acid on the plant weight and height, leaf area, photosynthetic pigments, and K+/Na+ ratio. Regarding the positive effect of potassium and ascorbic acid on the growth parameters along with the similar changes on the photosynthetic pigments and the K+/Na+ ratio with the reduction of total free amino acids, it may suggest both treatments improved plant growth through plant stability and increasing photosynthetic rate, production of more protein and other metabolites in Catharanthus roseus. Due to the importance of chemical compounds in Catharanthus roseus, any increase in the growth parameters to an increase in the yield of the plant can be very valuable.

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