Department of Horticultural Science, Islamic Azad University, Rasht Branch, Rasht, Iran
10.22034/ijpp.2020.672571
Abstract
A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in combination. A combination of 1.00 mg l-1 KIN + 1.00 mg l-1 IBA was found to be suitable for regeneration of most measured characteristics especially maximum PLBs regeneration (30.40/plantlet), leaf number (5.93/plantlet), and root number (8.36/plantlet) from protocorm explants. The maximum number of plantlets (11.66) was calculated on MS medium supplemented with 1.00 mg l-1 KIN + 0.50 mg l-1 IBA, followed by 1.00 mg l-1 KIN + 1.00 mg l-1 IBA (10.33). Plantlets were transplanted to pots filled with cocochips and sphagnum moss (70:30) for acclimatization and transferred to the greenhouse. Upon ex vitro transfer, 90% of plants survived.
Asa, M., Kaviani, B. (2020). In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa. Iranian Journal of Plant Physiology, 10(2), 3113-3123. doi: 10.22034/ijpp.2020.672571
MLA
Mozhdeh Asa; Behzad Kaviani. "In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa". Iranian Journal of Plant Physiology, 10, 2, 2020, 3113-3123. doi: 10.22034/ijpp.2020.672571
HARVARD
Asa, M., Kaviani, B. (2020). 'In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa', Iranian Journal of Plant Physiology, 10(2), pp. 3113-3123. doi: 10.22034/ijpp.2020.672571
VANCOUVER
Asa, M., Kaviani, B. In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa. Iranian Journal of Plant Physiology, 2020; 10(2): 3113-3123. doi: 10.22034/ijpp.2020.672571
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