Evaluation of proline content and enzymatic defense mechanism in response to drought stress in rice

Document Type : Original Article

Authors

1 Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

2 Department of Plant Production, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran

3 Nuclear Agriculture Group, Nuclear Science and Technology Research Institute, Karaj, Iran

10.22034/ijpp.2019.664580

Abstract

To study proline content and enzyme defense system in response to drought stress in rice, a randomized complete block design with three replications was conducted in two separate environments (drought stress and non-stress). The rice cultivars used included two commercial cultivars, i.e., Ahlemi Tarom (low-yield and drought tolerant) and Sepidrood (high-yield and sensitive to drought) and three promising lines of fourth generation mutants (high-yield and drought tolerant), namely, No. 4, 94 and 95 tested on a research farm at Gonbad Kavous University in the 2018 crop year. Plant roots were separately sampled in each block at the reproductive stage. The proline content and activity of the enzymes involved in the defense system, including superoxide dismutase, catalase, ascorbate peroxidase and glutathione peroxidase were measured by extracting the enzymes from each root sample. Results indicated that the drought-tolerant mutant lines had lower proline contents. The mutant lines and Ahlemi Tarom and Sepidrood cultivars had the highest to lowest levels of defense enzymes in the oxidative pathway, respectively. Correlation was found between the decrease in proline contents and increase in levels of defense enzymes of the oxidative stress pathway. The mutants possessed reliable genetic storages for tolerance to drought stress and had a significant superiority over the commercial cultivars at P≤0.05.
 

Keywords


Aebi, H. 1984. 'Catalase in vitro'. Methods in Enzymology, 105: 121-126.
Ahmadi, A. and A. Sio-Se Mardeh. 2004. 'The effects of water stress on soluble carbohydrates, chlorophyll and proline contents of four Iranian wheat cultivars under different moisture regimes'. Iranian Journal of Agriculture Science, 35(3): 753-763 (in Persian).
Amini, Z., R. Hadad and F. Moradi. 2009. 'The effect of water deficit stress on antioxidant enzymes during generative growth stages in Barley (Hordeumvulgare L.)'. Journal of Water and Soil Science, 12(46): 65-74 (in Persian).
Anne, C. L. and T. Bruno. 1997.' Glutathiowe-mediated regulation of ATP sulfurylase activity, , uptake, and oxidative stress response in lntact canola roots'. Plant Physiology, 114: 177-183.
Asada, K. 1990. 'Ascorbate peroxidase-a hydrogen peroxide-scavenging enzyme in plants'. Physiologia Plantarum, 85: 235-241.
Asada, K. 1999. 'The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons'. Annual Review of Plant Physiology and Plant Molecular Biology, 50: 601-639.
Asada, K. 2000. 'The water-water cycle as alternative photon and electron sinks'. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 355(1402): 1419-1431.
Ashraf, M. Y., A. R. Azmi, A. H. Khan and S. A. Ala. 1994. 'Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat'. Acta Physiologiae Plantarum, 16(3): 185-191.
Babaee, K., M. Amini Dehaghi, S. A. M. ModaresSanavi and R. Jabbari. 2010. 'Water deficit effect on morphology, proline content and thymol percentage of Thyme (Thymus vulgaris L)'. Iranian Journal of Medicinal and Aromatic Plants, 26(2): 239-251.
Bates, L. 1973. 'Rapid determination of free proline for water stress studies'. Plant and Soil, 39(1): 205-207.
Beauchamp, C. and I. Fridovich. 1971. 'Superoxide dismutase: improved assays and an assay applicable to acrylamide gels'. Analytical Biochemistry, 44(1): 276-287.
Bowler, C., M. Van Montagu and D. Inze. 1992. 'Superoxide dismutase and stress tolerance'. Annual Review of Plant Physiology and Plant Molecular Biology, 43: 83-116.
Bhattacharjee, S. 2005. 'Reactive oxygen species and oxidative burst: Roles in stress, senescence and signal transduction in plants'. Current Science, 89(7): 1113-1121.
Chance, B. and A. C. Maehly. 1955. 'Assay of catalase and peroxidase󠇦'. Methods Enzymology, 2: 764-775.
Choi, H. W., Y. J. Kim, S. C. Lee, J. K. Hong and B. K. Hwang. 2007. 'Hydrogen peroxide generation by the pepper extracellular peroxidase CaPO2 activates local and systemic cell death and defense response to bacterial pathogens'. Plant Physiology, 145(3): 890-904.
Du, Y. Y., P. C. Wang, J. Chen and C. P. Song. 2007. 'The comprehensive functional analysis of catalase gene family in Arabidopsis thaliana'. National Natural Science Foundation of China, 121(1):16-23.
Du, Y. Y., P. C. Wang, J. Chen and C. P. Song. 2008. 'Comprehensive functional analysis of the catalase gene family in Arabidopsis thaliana'. Journal of Integrative Plant Biology, 50(10): 1318-1326.
Esfandiari, E. and A. Javadi. 2014. 'Role of scavenging enzymes and hydrogen peroxide and glutathione S-transferase in mitigating the salinity effects on wheat'. Iranian Journal of Plant Biology, 6(20): 1-16 (in Persian).
Ettinger, W. F. and J. J. Harada. 1990. 'Translational or post-translational processes affect differentially the accumulation of isocitratelyase and malate synthase proteins and enzyme activities in embryos and seedlings of Brassica napus'. Archives of Biochemistry and Biophysics, 281(1): 139-143.
Farhoudi, R. and M. Makezadeh Tafti. 2012. 'Evaluation of drought stress effect on growth, yield, essential oil and chamazulene percentage of three chamomile (Matricaria recutita L.) cultivars in Khuzestan condition'. Iranian Journal of Field Crops Research, 10(4): 735-741. 
Foyer, C. H. and J. Harbinson. 1994. 'Oxygen metabolism and the regulation of photosynthetic electron transport. In C. H. Foyer, PM Mullineaux, eds, Causes of Photo-oxidative Stress and Amelioration of Defence System in Plants'. CRC Press, Boca Raton, FL, Pp: 1-42.
Foyer, C. H. and G. Noctor. 2005. 'Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses'. The Plant Cell, 17(7): 1866-1875.
Gautam, P. and J. Stein. 2011. 'Induction of systemic acquired resistance to Pucciniasorghi corn'. International Journal of Plant Pathology, 2: 43-50.
Ghogdi, E., A. Borzouei, S. Jamali and N. Hashempour. 2013. 'Changes in root traits and some physiological characteristics of four wheat genotypes under salt stress'. International Journal of Agriculture and Crop Sciences, 5: 838-844.
Giannopolitis, C. N. and S. K. Ries. 1977. 'Superoxide dismutase: I. Occurrence in higher plants'. Plant Physiology, 59: 309-314.
Golden, T. A., D. Hinerfeld and S. Melov. 2002. 'Oxidative stress and aging beyond correlation'. Aging Cell, 122: 117-123.
Halliwell, B. 2006. 'Reactive species and antioxidants: Redox biology is a fundamental theme of aerobic life'. Plant Physiology, 141(2): 312-322.
Herbette, S., C. Lenne, N. Leblanc, J. L. Julien, J. R. Drevet and P. Roeckel-Drevet. 2002. 'Two GPX-like proteins from Lycopersicon esculentum and Helianthus annuus are antioxidant enzymes with phospholipid hydroperoxide glutathione peroxidase and thioredoxin peroxidase activities'. European Journal of Biochemistry, 269(9): 2414-2420.
Hosseini, S. S., S. S. Ramezanpour, H. Soltanloo and S. Vakili Bastam. 2017. 'Evaluating expression of genes involved in proline synthesis pathway in mutant wheat T65-58-8 under drought stress'. Modern Genetics Journal, 12(3): 451-461 (in Persian).
Jung, B. G., K. O. Lee, S. S. Lee, Y. H. Chi, H. H. Jang and S. S. Kang. 2002. 'A Chinese cabbage cDNA with high sequence identity to phospholipid hydroperoxide glutathione peroxidases encodes a novel isoform of thioredoxin-dependent peroxidase'. The Journal of Biological Chemistry, 277(15): 12572-12578.
Kazerani, B., S. Navabpour, H. Sabouri, S. S. Ramezanpour, K. Zaynali Nezhad and A. Eskandari. 2018. 'Determination of the best selection indices in mutant lines of rice at different moisture conditions'. Crops Improvement, 20(1): 173-189 (in Persian).
Kazerani, B., S. Navabpour, H. Sabouri, S. S. Ramezanpour, K. Zaynali Nezhad and A. Eskandari. 2019. 'Evaluation and selection of rice mutant lines based on drought tolerance indices'. Journal of Plant Production Research, 25(4): 15-31 (in Persian).
Larkindale, J. D., J. R. Hall, M. Knight and E. Vierling. 2005. 'Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance'. Plant Physiology, 138: 882-897.
Levitt, J. 1980. 'Responses of plant to environmental stresses'. Vol.1, Academic press Inc.
Mackerness, S. A. H., B. R. Jordan and B. Thomas. 1999. 'Reactive oxygen species in the regulation of photosynthetic genes by ultraviolet-B radiation (UV-B: 280-320 mm) in green and etiolated buds of pea (Pisum sativum L.)'. Journal of Photochemistry and Photobiology, 48: 180-188.
Maiorino, M., C. Gregolin and F. Ursini. 1990. 'Phospholipid hydroperoxide glutathione peroxidase'. Method in Enzymology, 186: 448-457.
Mazandarani. A., M. Rahim Malek, S. Navabpour and S. S. Ramezanpour. 2014. 'Evaluation of chlorophyll content and genes expression (Catalase and DREB1) in soybean cultivars under drought stress condition'. Agricultural Biotechnology, 5(1): 45-58 (in Persian).
Mirza Masoumzadeh, B., A. A. Imani and S. Khayamaim. 2012. 'Salinity stress effect on proline and chlorophyll rate in four beet cultivars'. Scholars Research Library, 3(12): 5453-5456.
Mittler, R. 2002. 'Oxidative stress, antioxidants and stress tolerance'. Trends in Plant Science, 7 (9): 405-410.
Nakano, Y. and K. Asada. 1981. 'Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts'. Plant & Cell Physiology, 22(5): 867-880.
Nakano, Y. and K. Asada. 1987.' Purification of ascorbate peroxidase in spinach chloroplast in inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical'. Plant & Cell Phsiology, 28: 131-133.
Navabpour, S. 2012. 'Effect of abscisic acid application on enzymatic and nonenzymatic activity in barley (Hordeum vulgare L.)'. Cereal Research, 1(1): 39-51 (in Persian).
Navabpour, S. 2013. 'Induced genes expression pattern in response to drought stress in Rapeseed (Brassica napus)'. Seed and Plant Improvement Journal, 1-29(3):535-549 (in Persian).
Navabpour, S., S. Mirkarimi and A. Mazandarani. 2013. 'Evaluation of enzymatic and non-enzymatic defense mechanism in response to Charcoal Rot disease during growth stage in soybean'. Crop Biotechnology, 3(5): 63-73 (in Persian).
Navabpour, S., S. S. Ramezanpour and A. Mazandarani. 2016. 'Evaluation of enzymatic and non-enzymatic defense mechanism in response to drought stress during growth stage in soybean'. Plant Production Technology, 7(2): 39-54 (in Persian).
Neill, S. J., R. Desikan, A. Clarke, R. Hurst and J. T. Hancock. 2001. 'Hydrogen peroxide and nitric oxide as signaling molecules in plants'. Journal of Experimental Botany, 52: 9-17.
Nickel, R. S. and B. A. Cunningham. 1969. 'Improved peroxidase assay method using Ieuco 2,3,6-trichlcroindophenol and application to comparative measurements of peroxidase catalysis'. Analytical Biochemistry, 27(2): 292-299.
Ozturk, L., Y. Demir, A. Unlukara, I. Karatas, A. Kurunc and O. Duzdemir. 2012.' Effects of long-term salt stress on antioxidant system, chlorophyll and proline contents in pea leaves'. Romanian Biotechnological Letters, 17(3): 7227-7236.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Pitzschke, A., C. Forzani and H. Hirt. 2006. 'Reactive oxygen species signaling in plants'. Antioxidants & Redox Signalling, 8(9-10): 1757-1764.
Yang, T. and B. W. Poovaiah. 2002. 'Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin'. Proceedings of National academy of Science of US (PNAS) 99: 4097-4102.
Yoshida, S. 1981.' Fundamentals of rice crop science'. International Rice Research Institute, Los Banos, Laguna, Philippines, 269p.