Efficiency of selenium and salicylic acid protection against salinity in soybean

Document Type : Original Article

Authors

1 Department of Biology,Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology, Garmsar Branch,Islamic Azad University, Garmsar, Iran

10.22034/ijpp.2019.664577

Abstract

This study was carried out in order to minimize risks associated with salinity. Soybean seedlings were treated with salicylic acid (SA) of 0 and 0.5 mM, selenium (Se) at 0, 25, and 50 mgl-1, and/or NaCl, 0 and 100 mM. The supplements promoted shoot fresh mass and markedly relieved toxicity signs of salinity. Se and/or SA increased calcium and potassium contents in different organs and mitigated the inhibiting effects of salinity on the nutritional status. Simultaneous applications of these elicitors were the most effective strategy to induce antioxidant enzymes (peroxidase, polyphenol oxidase, and catalase). Also, these compounds increased proline, phenolics, and soluble carbohydrates in leaves and roots. Activities of phenylalanine ammonia lyase in roots and leaves were induced in the Se and/or SA-supplemented plants. In addition, the elicitors triggered accumulations of non-protein thiols. Therefore, the simultaneous application of Se and SA may be considered as a promising way to relieve the toxicity signs of salinity.

Keywords


An, C., and Z. Mou, 2011. 'Salicylic acid and its function in plant immunity'. Journal of Integrative Plant Biology 53: 412-428.
Ardebili, N.O., S. Saadatmand, V. Niknam and R.A., Khavari-Nejad. 2014. 'The alleviating effects of selenium and salicylic acid in salinity exposed soybean'. Acta Physioliogia Plantarum, 36: 3199-205.
Ardebili, Z.O. and M. Moradi. 2016. 'The modification in quality of parsley (Petroselinum crispum) by selenium and amino acids'. Iranian Journal of Plant Physiology, 6(4): 1825-1833.
Ardebili, Z.O., N.O. Ardebili, S. Jalili and S., Safiallah. 2015.' The modified qualities of basil plants by selenium and/or ascorbic acid'. Turkish Journal of Botany, 39: 401-407.
Asgari-Targhi, G., A. Iranbakhsh and Z. O. Ardebili. 2018. 'Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micropropagation of Capsicum annuum.' Plant Physiology and Biochemistry, 127: 393-402.
Babajani, A., A. Iranbakhsh, Z.O. Ardebili and B. Eslami. 2019. 'Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles: cold plasma as a novel emerging tool for plant science'. Plasma Chemistry and Plasma Processing, 39(1): 21-34.
Bates, L., R. Waldren and I. Teare. 1973. 'Rapid determination of free proline for water-stress studies'. Plant and Soil, 39: 205-207.
Beaudoin-Eagan, L.D. and T. Thorpe .1985. 'Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures'. Plant Physiology, 78:438-441.
Bitterli, C., G. Bañuelos and R. Schulin. 2010.' Use of transfer factors to characterize uptake of selenium by plants'. Journal of Geochemical Exploration, 107: 206-216.
Bradford, M.M. 1976. 'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding'. Analytical Biochemistry,72: 248-254.
Çelik, Ö. and S.G. Ünsal. 2013. 'Expression analysis of proline metabolism-related genes in salt-tolerant soybean mutant plants'. Plant Omics, 6(5):364-370.
Cheng, B., H.F. Lian, Y. Liu, X. Yu, Y. Sun, X. Sun, Q. Shi and S.Q. Liu. 2016. 'Effects of selenium and sulfur on antioxidants and physiological parameters of garlic plants during senescence'. Journal of Integrative Agriculture, 15: 566-572.
Del Longo, O.T., C. González, G. Pastori and V.S. Trippi. 1993. 'Antioxidant defenses under hyperoxygenic and hyperosmotic conditions in leaves of two lines of maize with differential sensitivity to drought'. Plant and cell physiology, 34: 1023-1028.
Feng, R., C. Wei and S. Tu. 2013. 'The roles of selenium in protecting plants against abiotic stresses'. Environmental and Experimental Botany, 87:58-68.
Golob A., J. Kavčič, V. Stibilj, A. Gaberščik, K. Vogel-Mikuš and M. Germ. 2017. 'The effect of selenium and UV radiation on leaf traits and biomass production in Triticum aestivum L.' Ecotoxicology and Environmental Safety, 136:142-149.
Guo, B., C. Liu, H. Li, K. Yi, N. Ding, N. Li, Y. Lin and Q. Fu. 2016. 'Endogenous salicylic acid is required for promoting cadmium tolerance of Arabidopsis by modulating glutathione metabolisms'. Journal of Hazardous Materials, 316: 77-86.
Hemeda, H. M. and B. P. Klein. 1990.' Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts'. Journal of Food Science, 55: 184-185.
Iranbakhsh, A., N.O. Ardebili, Z.O. Ardebili, M. Shafaati, and M. Ghoranneviss. 2018a. 'Non-thermal plasma induced expression of heat shock factor A4A and improved wheat (Triticum aestivum L.) growth and resistance against salt stress'. Plasma Chemistry and Plasma Processing, 38(1): 29-44.
Iranbakhsh, A., Z. O. Ardebili, N.O. Ardebili, M. Ghoranneviss, and N. Safari. 2018b. 'Cold plasma relieved toxicity signs of nano zinc oxide in Capsicum annuum cayenne via modifying growth, differentiation, and physiology'. Acta Physiologiae Plantarum, 40(8):154.
Ježek, P., J. Hlušek, T. Lošák, M.  Jůzl, P.  Elzner, S. Kráčmar, F. Buňka and A. Martensson. 2011. 'Effect of foliar application of selenium on the content of selected amino acids in potato tubers (Solanum tuberosum L.)'. Plant Soil and Environment, 57: 315-320.
Loutfy, N., M. El-Tayeb, A.  Hassanen, M. Moustafa, Y. Sakuma and M. Inouhe. 2012. 'Changes in the water status and osmotic solute contents in response to drought and salicylic acid treatments in four different cultivars of wheat (Triticum aestivum)'. Journal of Plant Research,125: 173-184.
Liu, X., Y. Yang, X. Deng, M. Li, W. Zhang and Z. Zhao. 2017. 'Effects of sulfur and sulfate on selenium uptake and quality of seeds in rapeseed (Brassica napus L.) treated with selenite and selenate'.  Environmental and Experimental Botany, 135: 13-20.
Nazar, R., N. Iqbal, S. Syeed and N.A. Khan, 2011. 'Salicylic acid alleviates decreases in photosynthesis under salt stress by enhancing nitrogen and sulfur assimilation and antioxidant metabolism differentially in two mungbean cultivars'. Journal of Plant Physiology, 168: 807-815.
Nazerieh, H., Z. Oraghi Ardebili and A. Iranbakhsh. 2018. 'Potential benefits and toxicity of nanoselenium and nitric oxide in peppermint'. Acta Agriculturae Slovenica, 111(2):357 – 368.
Pereira, G., S. Molina, P. Lea and R. Azevedo. 2002. 'Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea'. Plant and Soil, 239: 123-132.
Raymond, J., N. Rakariyatham and J. Azanza. 1993.' Purification and some properties of polyphenoloxidase from sunflower seeds'. Phytochemistry, 34: 927-931.
Safari, M., Z. Oraghi Ardebili and A. Iranbakhsh, 2018. 'Selenium nano-particle induced alterations in expression patterns of heat shock factor A4A (HSFA4A), and high molecular weight glutenin subunit 1Bx (Glu-1Bx) and enhanced nitrate reductase activity in wheat (Triticum aestivum L.)'. Acta Physiologiae Plantarum, 40: 117-124.
Seppänen, M., M. Turakainen and H. Hartikainen. 2003. 'Selenium effects on oxidative stress in potato'. Plant Science, 165: 311-319.
Sun, H., H. Dai, X. Wang and G. Wan. 2016. 'Physiological and proteomic analysis of selenium-mediated tolerance to Cd stress in cucumber (Cucumis sativus L)'. Ecotoxicology and Environmental Safety, 133:114-126.
Szabados, L. and A. Savouré. 2010. 'Proline: a multifunctional amino acid'. Trends in Plant Sciences, 15: 89-97.
Tamaoki, M., J.L. Freeman and E.A. Pilon-Smits. 2008. 'Cooperative ethylene and jasmonic acid signaling regulates selenite resistance in Arabidopsis'. Plant Physiology, 146: 1219-1230.
Wasti, S., H. Mimouni, S. Smiti, E. Zid and H. Ben Ahmed. 2012.' Enhanced salt tolerance of tomatoes by exogenous salicylic acid applied through rooting medium'. Omics: A Journal of Integrative Biology, 16: 200-207.
Wu, Z., S. Liu, J. Zhao, F. Wang, Y. Du, S. Zou, H. Li, D. Wen and Y. Huang. 2017. 'Comparative responses to silicon and selenium in relation to antioxidant enzyme system and the glutathione-ascorbate cycle in flowering Chinese cabbage (Brassica campestris L ssp chinensis var utilis) under cadmium stress'. Environmental and Experimental Botany, 133:1-11.
Yusuf, M., T.A. Khan and Q. Fariduddin. 2016. 'Interaction of epibrassinolide and selenium ameliorates the excess copper in Brassica juncea through altered proline metabolism andantioxidants'. Ecotoxicology and Environ
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
mental Safety, 129: 25-34.
Zagorchev, L., C.F. Seal, I. Kranner and M. Odjakova. 2013. 'A central role for thiols in plant tolerance to abiotic stress'. International Journal of Molecular Sciences, 14: 7405-7432.
Zhu, Z., Y. Chen, G. Shi and X. Zhang. 2017. 'Selenium delays tomato fruit ripening by inhibiting ethylene biosynthesis and enhancing the antioxidant defense system'. Food Chemistry, 219:179-184.