Enhancement of valerenic acid production in Valeriana officinalis roots by methyl jasmonate -mediated transcriptional changes of sesquiterpene synthase genes

Document Type : Original Article

Authors

1 1Assistant Professor, Department of Agricultural Biotechnology, Ramin Agriculture & Natural Resources University of Khouzestan, Iran.

2 Department of Agronomy and Plant Breeding, Ramin Agriculture & Natural Resources University of Khouzestan, Iran

10.22034/ijpp.2018.545660

Abstract

Valeriana officinalis (valerian), as a nutraceutical herb, is widely used for its sedative and hypnotic properties. It is known that C15 sesquiterpenoid valerenic acid (VA) is the active ingredient responsible for pharmacological effects of V. officinalis. To evaluate the effect of methyl jasmonate (MeJA) concentrations (50 and 100 µM) in the modulation of expression patterns of the genes involved in valerenic acid (VA) biosynthesis, transcript abundance of identified sesquiterpene synthase (Sesqui-TPS) genes in the root of V. officinalis was monitored by quantitative real time PCR (qRT-PCR) within a 144 h time period. In addition, Valerenic acid contents were measured by high-performance liquid chromatography (HPLC). The highest amount of VA (12.45 mg/g dry weight (DW)) was found at 100 µM MeJA with a 12 fold increase over control culture (1.03 mg/g DW) at exposure time of 72 h. Moreover, MeJA in a concentration dependent manner enhanced transcription rate of VoTPS1 and VoTPS7 genes. Accordingly, exposure to 100 µM MeJA for 24 h can be more effective in the induction of these genes than that observed for 50 µM. Such enhancement was correlated with increased VA accumulation suggesting that these genes may be responsible for the biosynthesis of intermediates involved in the VA-biosynthetic pathway. However, MeJA treatment seemed to have a less significant effect on VoTPS3 expression than VoTPS1 and VoTPS7 genes. This results provide insights for more effective biosynthesis of VA by MeJA-mediated transcriptional changes of putative sesqui-TPS.

Keywords

References

Afrin, S., J.J. Huang and Z.Y. Luo, 2015. 'JA-mediated transcriptional regulation of secondary metabolism in medicinal plants'. Science Bulletin, 60(12):1062–1072.
Beaubru, G and G.E. Gray, 2000. 'A review of herbal medicines for psychiatric disorders'. Psychiatric Services ,51: 1130-1134.
Bent, S., A. Padula, D. Moore, M. Patterson andW. Mehling, 2006. 'Valerian for sleep: a systematic review and meta-analysis'. The American Journal of Medicine, 119: 1005-1012.
Chico, J.M., A. Chini, S. Fonseca and R. Solano, 2008. ,'AZ repressors set the rhythm in jasmonate signaling'. Current Opinion in Plant Biology, 11(5):486-94.
Chini, A., S. Fonseca, G. Fernandez, B. Adie , J.M. Chico, O. Lorenzo, G. García-Casado,  I. López-Vidriero, F.M. Lozano, M.R. Ponce, J.L. Micoland R. Solano, 2007. 'The JAZ family of repressors is the missing link in jasmonate signalling'. Nature, 448:666–671
DiniTorkamani, MR., M. Jafari, N. Abbaspour and N. Safaie, 2014. 'Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation'. Central European Journal of Biology, 9(9): 853–863
Endt, DV., J.W. Kijne and J. Memelink, 2002. 'Transcription factors controlling plant secondary metabolism: what regulates the regulators?'. Phytochemistry, 61: 107–114.
Hampel, D., A. Mosandl and M. Wust, 2005. 'Biosynthesis of mono- and sesqui-terpenes in carrot roots and leaves (Daucus carota L.): metabolic cross talk of cytosolic mevalonate and plastidial methylerythritol phosphate pathway'. Phytochemistry, 66: 305-311.
Letchamo, W., W. Ward, B. Heard and D. Heard, 2004. 'Essential oil of Valeriana officinalis L. cultivars and their antimicrobial activity as influenced by harvesting time under commercial organic cultivation'. Journal of Agricultural and Food Chemistry, 52: 3915-3919.
Livak, K.J and T.D. Schmittgen, 2001. 'Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method'. Methods, 25: 402-408.
Memelink, J., 2009. 'Regulation of gene expression by jasmonate hormones'. Phytochemistry, 70: 1560–1570.
Morazzoni, P and E. Bombardelli, 1995. 'Valeriana officinalis: traditional use and recent evaluation of activity'. Fitoterapia, 66: 99-112.
Murashige, T and F. Skoog, 1962. 'A revised medium for rapid growth and bioassays with tobacco tissue cultures'. Plant Physiology, 15: 473-497.
Pandey, S. S., S. Singh, C.S. Babu, K. Shanker, N.K. Srivastava, A.K. Shukla, 2016. 'Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis'. Scientific Reports, 6:26583.
Pfaffl M.W., 2001. 'A new mathematical for relative quantification in real time RT-PCR'. Nucleic acids Research, 29:e45.
Santos, MS., F. Ferreira, A.P. Cunha, A.P. Carvalho and T. Macedo, 1994. 'An aqueous extract of valerian influences the transport of GABA in synaptosomes'. Planta Medica, 60: 278-279.
Takemoto, H., T. Yagura and M. Ito, 2009. 'Evaluation of volatile components from spikenard. Valerena 4.7(11).diene is a highly active sedative compound'. Journal of Natural Medicines, 63: 380-385.
Tania, L and C.F. Brian, 2004. 'In vitro activity of commercial valerian root extracts against human cytochrome P450 3A4'. Journal of Pharmaceutical Sciences, 7: 265-273.
 Uddin, MR., A.A. Thwe, Y.B. Kim, W.T. Park, S.C. Chae, and S.U. Park, 2013. 'Effects of Jasmonates on Sorgoleone Accumulation and Expression of Genes for Sorgoleone Biosynthesis in Sorghum Roots'. Journal of Chemical Ecology, 39(6): 712–722.
Wang, Y., L. Jin, S. Yu, Q. Shi, Y. Gu and H. Kiyota, 2010. 'Chemical constituents of plants from the genus Valeiana'. Mini-Review in Organic Chemistry, 7: 161-172.
Yang, Z., B. Patra, R. Li, S. Pattanaik and L. Yuan, 2013.' Promoter analysis reveals cis-regulatory motifs associated with the expression of the WRKY transcription factor CrWRKY1 in Catharanthus roseus'. Planta, 238(6):1039-49
Yeo, YS., S.E. Nybo, A.G. Chittiboyina, A.D. Weerasooriya, Y.H. Wang, E. Góngora-Castillo, B. Vaillancourt, C.R. Buell, D. DellaPenna, M.D. Celiz et al., 2013. 'Functional identification of valerena-1,10-diene synthase, a terpene synthase catalyzing a unique chemical cascade in the biosynthesis of biologically active sesquiterpenes in Valeriana officinalis'. Journal of Biological Chemistry, 288: 3163–3173.
Zhang, L., R.X. Ding, Y.R. Chai, M. Bonfill, E. Moyano et al., 2004. 'Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures'. Proceedings of the National Academy of Sciences, 101: 6786–6791.
 
Iranian Journal of Plant Physiology
Volume 9, Issue 1
Autumn 2018
Pages 2611-2617

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