In the present study, dormancy and germination requirements were investigated in seeds of Citrullus colocynthis (L.). Seeds were subjected to 10 pretreatments in the present study.These pretreatments included hot water (100° C) followed by the seeds placed between double layered filter paper moistened with 5 ml of distilled water and kept at 28 - 30 °C, sulphuric acid (98% v/v), for 30 min followed by theseedsplaced between double layered filter paper moistened with 5 ml of distilled water and kept at 28 – 30° C, and sulphuric acid (98% v/v), for 5, 10, 15, and 20 min after which seeds were placed between double layered filter paper moistened with 5 ml of distilled water and kept at alternating temperatures 20 / 25° C (12h / 12h). In the last four pretreatments, Citrullus colocynthis (L.) seeds were subjected to10 and 20 mg/l GA3 followed by moistening with 5 ml of distilled water, 10 mg/l GA3, or 20 mg/l GA3. Concentrated sulphuric acid was partly-successful in dormancy-breaking. Moreover, germination increased at pretreatments and treatments of various levels of gibberellic acid. The highest germination rate and percentage were obtained at concentration of 10 mg/l GA3.