• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter
Plant Physiology
arrow Articles in Press
arrow Current Issue
Journal Archive
Volume Volume 9 (2019)
Volume Volume 8 (2018)
Volume Volume 7 (2017)
Volume Volume 6 (2016)
Volume Volume 5 (2015)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 4 (2014)
Volume Volume 3 (2013)
Volume Volume 2 (2012)
Volume Volume 1 (2011)
(2015). Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Elahe Baharan1, Payam Pour Mohammadi1, Ehsan Shahbazi2 and Seyedeh Zahra Hosseini3. Plant Physiology, 5(4), 1473-1481. doi: 10.22034/ijpp.2015.539675
. "Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Elahe Baharan1, Payam Pour Mohammadi1, Ehsan Shahbazi2 and Seyedeh Zahra Hosseini3". Plant Physiology, 5, 4, 2015, 1473-1481. doi: 10.22034/ijpp.2015.539675
(2015). 'Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Elahe Baharan1, Payam Pour Mohammadi1, Ehsan Shahbazi2 and Seyedeh Zahra Hosseini3', Plant Physiology, 5(4), pp. 1473-1481. doi: 10.22034/ijpp.2015.539675
Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Elahe Baharan1, Payam Pour Mohammadi1, Ehsan Shahbazi2 and Seyedeh Zahra Hosseini3. Plant Physiology, 2015; 5(4): 1473-1481. doi: 10.22034/ijpp.2015.539675

Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Elahe Baharan1, Payam Pour Mohammadi1, Ehsan Shahbazi2 and Seyedeh Zahra Hosseini3

Article 4, Volume 5, Issue 4, Summer 2015, Page 1473-1481  XML PDF (773.97 K)
Document Type: Original Article
DOI: 10.22034/ijpp.2015.539675
Abstract
The present study was carried out to evaluate the effects of light and plant growth regulators on callus induction and explant browning in date palm cultivar, Estameran in in vitro condition. The explants were pretreated by an antioxidant combination (100 mgl-1 citric acid and 150 mgl-1 ascorbic acid) for 3 days in 5 °C. Then, the  leaf explants  were cultured in Murashige and Skoog medium (MS) with 3 gL-1 of activated charcoal, 75 mgl-1 citric acid and 75 mgl-1 ascorbic acid, for 14 days. After that, explants cultured in callogenesis medium were supplemented with 12 different levels of plant growth regulators including 2,4-D (0 and 5 mgl-1), TDZ (0 and 5 mgl-1), and  BAP at three concentrations (0, 5, and 10 mgl-1), and two incubation conditions (light condition and absolute darkness).  The number of explants that induced callus and browning was recorded after six months. Results indicated that 2,4-D and BAP increased browning and this was exacerbated under light condition. Highest callus induction was achieved in medium supplemented with 5 mgl-1 BAP or with 5 mgl-1 TDZ. This experiment showed that low concentrations of cytokinin induced callus in date palm in vitro leave culture and callus induction increased in darkness.
Keywords
Adult leaf explants; callogenesis; date palm; light and dark
Statistics
Article View: 64
PDF Download: 98
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

Journal Management System. Designed by sinaweb.